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点击次数：2783 发布时间：2020/5/19 10:30:26
二、培养条件：
培养细胞名称：兔前脂肪细胞(preadipocytes)
培养体系：6孔板
培养细胞数量：6 × 105
三、用zeta life胎牛血清培养前脂肪细胞(preadipocytes)发表文章部分内容：
Isolation ａnd induction of rabbit preadipocytes Visceral preadipocytes were isolated from perirenal adipose tissue of newborn New Zealand rabbits under sterile condi[1]tions. Briefly, adipose tissues were digested with 0.01% collagenase I (Gibco, Carlsbad, CA, USA). Then, preadipocytes were seeded into 6-well plates at a density of 6 × 105 cells per plate in complete medium (DME/F12, sup[1]plemented with 10% fetal bovine serum [FBS]) (DME/F12 was obtained Gibco, Carlsbad, CA, USA; fetal bovine serumwas from Zeta life, Menlo Park, CA, USA), and preadipocyteswere cultured in a humidified incubator at 37 °C ａnd 5% CO2. Upon reaching confluency (set to day 0), cells were induced by the addition of differentiation medium I (DME/F12 with 1 μM dexamethasone, 500 μM 1-methy1-3-iosbutylxanthine, 1.7 μM insulin, 10% FBS) (dexamethasone, 1-methy1-3- iosbutylxanthine, ａnd insulin were from Solarbio, Beijing, China) for 72 h (day 3). Next, cells were cultured with differ[1]entiation medium II (DME/F12, 1.7 μM insulin, 10% FBS) for an additional 72 h ａnd further cultured in complete medi[1]um until day 9 (day 9). To identify lipid accumulation in cells and obtain mature adipocytes, the accumulation of lipid drop[1]lets was measured by Oil Red O staining. Based on three different phenotypes of lipid accumulation (almost no lipid droplets, a small amount of ring-shaped lipid droplets, ａnd a cluster of bigger lipid droplets), a total of nine cell samples were collected, ａnd each interval time point contained three biological replicates. RT-qPCR was performed to determine the mRNA expression levels of adipogenic marker genes, in[1]cluding PPARG, CEBPA, and FABP4 (primer sequences are shown in Table S1), ａnd the 2-ΔΔCt method was used to ana[1]lyze the relative expression. The housekeeping gene GAPDH served as control.
zeta life胎牛血清信息如下
| 产品名称 | 货号 | 规格 | 数量 |
| Fetal Bovine Serum France Origin | Z7186FBS-500 | 500ml | 1 |
| Fetal Bovine Serum France Origin | Z7186FBS-100 | 100ml | 1 |
| Fetal Bovine Serum ,South America Origin | Z7181FBS-500 | 500ml | 1 |
| Fetal Bovine Serum ,South America Origin | Z7181FBS-100 | 100ml | 1 |
| Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin | Z7180FBS-500 | 500ml | 1 |
| Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin | Z7180FBS-100 | 100ml | 1 |
| Fetal Bovine Serum (Australia Origin） | Z7010FBS-500 | 500ml | 1 |
| Fetal Bovine Serum (Australia Origin） | Z7010FBS-100 | 100ml | 1 |
| Fetal Bovine Serum ,South America Origin | Z7185FBS-500 | 500ml | 1 |
| Fetal Bovine Serum ,South America Origin | Z7185FBS-100 | 100ml | 1 |
原创作者：上海创凌生物科技有限公司
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