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阅读次数:2950 发布时间:2020/6/15 8:56:43
一、2020季度,清华大学免疫学与医学院;清华大学生命科学学院联合发表标题为:The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens的文章到JEM;
二、用invigentech(英克)INVI DNA RNA Transfection Reagent转染试剂;
三、成功转染BMDMs细胞(小鼠骨髓来源的巨噬细胞)发表文章见刊;
四、文章简介:结肠巨噬细胞转录因子RBP-J协调肠道对细菌的免疫病原体研究.
五、文章部分内容见下:
RNA-mediated interference
siRNA specifically targeting mouse Cebpb and nontargeting control siRNA were purchased from GenePharma Company. siRNA was transfected into BMDMs using INVI DNA RNA Transfection Reagent according to the manufacturer’s in structions (Invigentech). Cells were used 48 h after transfection.