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MEGAscript™ T7 Transcription Kit 试剂盒现货促销

MEGAscript™ T7 Transcription Kit 试剂盒现货促销

价格:电议

采购度:742    原产地:中国大陆

发布时间:2018/3/7 14:28:58

MEGAscript™ T7 Transcription Kit 试剂盒现货促销,The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit.

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标签:Transcription   MEGAscript™      试剂盒促销   

详细信息
MEGAscript™ T7 Transcription Kit 试剂盒现货促销产品资料
 

品牌:

Thermo-life

货号:

AM1333

 

供应商:

上海创凌生物科技有限公司

 

数量:

充足

 

保质期:

三年

 

保存条件:

低温

 

规格:

EACH

 

产品名称:MEGAscript™ T7 Transcription Kit

MEGAscript™ T7 Transcription Kit 试剂盒现货促销产品特点

The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.

Using the MEGAscript® T7 Kit
The MEGAscript® T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 µg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.
                                                                                                                                                                                      
Applications
The MEGAscript® T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. The MEGAscript® T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.

Accessory products
MessageAmp™ aRNA amplification kits are powered by MEGAscript® technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitrotranscription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).

MEGAscript™ T7 Transcription Kit 试剂盒现货促销产品规格

Starting Material:

Linearized DNA, PCR Products

Promoter:

T7

RNA Yield:

100 µg

Final Product:

In Vitro Transcribed RNA

Product Line:

Ambion™, MEGAscript®

Product Size:

25 reactions

Shipping Condition:

Dry Ice

                                                                                                                                                              

MEGAscript™ T7 Transcription Kit 试剂盒现货促销注意事项
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate S Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

MEGAscript™ T7 Transcription Kit 试剂盒现货促销,畅销国内外,欢迎新客户来电咨询我司产品,创凌生物将以卓越的品质和周到的服务竭诚为您服务。

更新时间:2024/1/4 13:19:42

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